ISAC

Program

  • FRIJune 5
  • SATJune 6
  • SUN June 7
  • MON June 8
  • TUE June 9
  • WED June 10
Friday, June 5

Pre-conference Course: The Fundamentals of Flow Cytometry — Principles & Applications

Meeting Room 1E

Derek Davies, Cytometry Consultant, UK -
Rachael Walker, Head of Flow Cytometry, Babraham Institute, Cambridge, UK -

*Pre-registration is required
Learn more here - Pre-Conference Courses

Flow cytometry is a widespread technique in the biomedical research world. It is a single cell technology capable of measuring multiple fluorescent analytes on thousands of cells per second. To design, run and analyse a successful flow cytometry experiment a researcher needs to know something about the fluorescent reagents that are used, how the cytometer works, how to design an experiment, how to prepare samples, and how to analyse them. This day long course will introduce delegates to all these aspects as well as illustrating some of the applications that flow cytometry is used for. Delegates will also take part in interactive exercises designed to consolidate their learning.

Pre-conference Course: Translational Flow Cytometry Course

Meeting Room 1F

Thomas Beadnell, Eurofins Viracor BioPharma Services -
Katharine Schwedhelm, Fred Hutch -
Nithianandan Selliah, Cerba Research -

*Pre-registration is required
Learn more here - Pre-Conference Courses

This course will present information regarding the best practices for conducting flow cytometric methods to support translational studies (clinical trials conducted in academic labs, pharmaceutical company labs or CRO’s) or to support clinical testing (CAP/CLIA or ISO 15189 laboratories). Instrument setup, method validation aligned with CLSI H62, data review and reporting will be covered.

Saturday, June 6

First Time Attendee Orientation

Meeting Room 2C

Attending the ISAC Annual Meeting (CYTO) for the first time? Join us for an engaging First-Timers Orientation, designed to help you navigate the conference, connect with peers, and make the most of your experience.

This session will introduce you to key sessions, networking opportunities, and insider tips to optimize your time at CYTO. Whether you're looking to explore cutting-edge cytometry research, engage with experts in the field, or expand your professional network, this orientation will provide the tools and insights to ensure a successful and rewarding conference.

What to Expect:
Overview of the ISAC community and CYTO structure
Guidance on sessions and events
Tips for networking and engaging with experts
Q&A with experienced ISAC members and past attendees
Start your CYTO journey with confidence—we look forward to welcoming you!

10:15am - 11:30am
Scientific Tutorials
11:45am - 1:00pm
Scientific Tutorials
1:00pm - 2:30pm
SRL Forum
2:30pm - 3:45pm
Scientific Tutorials
4:00pm - 5:00pm
Workshops

Career Pathways Panel Discussion

Meeting Room 2C

Hosted by CYTO Women

5:00pm - 5:30pm
Break
Grand Ballroom Foyer

CYTO Innovation and Technology Showcase

Grand Ballroom

A fast-paced, entertaining, and informative showcase, which will provide exposure and new opportunities to some of the most exciting new companies in cytometry.The three finalists will present their technology and business pitches. They will be questioned by a panel of judges with expertise in the scientific aspects of cytometry, but also the business techniques required for the success of a young innovative company.

President's Reception

By Invitation Only

Sunday, June 7

Opening and State-of-the-Art

Grand Ballroom

From Big Data to Good Data: How High-Performance Imaging Drives Biomedical Breakthroughs

Keisuke Goda, PhD, Professor, University of Tokyo -

AI has rapidly advanced in biology and medicine, demonstrating value in areas such as drug discovery, patient monitoring, and personalized healthcare. Yet, the performance and reliability of AI ultimately depend on the underlying data. Regardless of how sophisticated an algorithm may be, practical clinical implementation is impossible without training datasets of sufficient quality and scale. In this talk, I will introduce a suite of innovative volumetric imaging technologies developed to generate high-quality, high-volume biological data. Through these technologies, I will highlight how high-performance imaging enables data-driven discovery and discuss its central role in realizing clinically deployable AI in biology and medicine.

Hematopoietic stem cells across the human lifespan: functional heterogeneity decoded by flow cytometry

Stephanie Xie, PhD, Scientist, Princess Margaret Cancer Centre, UHN -

Hematopoietic stem cells (HSC) have enormous regeneration capacity (~10E11 cells daily in the human). Adult humans are estimated to have 50,000 to 200,000 HSC contributing to hematopoiesis at any one time. Understanding the functional diversity of human HSC across a lifetime is crucial for promoting healthy aging, advancing medicine, and improving therapeutic strategies for hematological disorders. Flow cytometry has long been a cornerstone for defining HSC populations, yet few immunophenotypic stem cell markers have been identified to link molecular signatures and functional outcomes. In this lecture, we present an integrated workflow that combines single-cell index sorting with downstream functional assays to systematically interrogate human HSC across ontogeny. We present a refined marker set—ATP2B1 and CD49f— to characterize long-term HSC from fetal liver, neonatal, and adult sources. ATP2B1 is a calcium exporter that enabled isolation of the most functionally superior population of human HSC described to date. ATP2B1⁺CD49f⁺ cells exhibit superior long-term repopulation and enrichment for endo-lysosomal pathways. We will discuss the workflow, key technical considerations, data integration strategies, and insights gained into the hierarchical organization and mechanistic regulation of human HSC function gained from multi-dimensional flow cytometry coupled to single cell differentiation assays and xenotransplantation. This platform provides a powerful framework for dissecting stem cell biology at unprecedented resolution and uncovering novel biomarkers and therapeutic targets in normal and malignant human hematopoiesis.

Jessica P. Houston, PhD, Professor, New Mexico State University -
10:00am - 10:30am
Coffee Break
Grand Ballroom Foyer
10:30am - 11:30am
Parallel Sessions
12:00pm - 1:00pm
Commercial Tutorials

Label-Free & Autofluorescence Cytometry Plenary

Grand Ballroom

Autofluorescence lifetime flow cytometry of immune cells

Melissa Skala, PhD, Professor, Morgridge Institute for Research -

The autofluorescence lifetimes of the metabolic co-enzymes NAD(P)H provide a label-free, functional readout of single-cell metabolism, which is sensitive to cell activation states and drug-induced metabolic changes. We developed a microfluidic flow cytometer that measures NAD(P)H autofluorescence decays from individual cells using time-correlated single-photon counting (TCSPC), with picosecond resolution and real-time phasor-based classification. This system combines a cost-effective pulsed UV diode laser excitation (50 MHz pulse repetition rate with short pulses ≤90ps), alkali photomultiplier tubes, and an FPGA-based time tagger for real-time analysis. We report continuous acquisition with a 100% duty cycle using on-chip decay histogramming, throughput of up to 100 cells per second with an average of 10,000 emission photons from each cell while receiving a safe excitation light dose (2.65 J/cm2 at 375nm), and microfluidic chip designs featuring vertical and horizontal sheath flow for cell focusing. We incorporate a second detection channel for far-red antibody markers to relate cell function on a single-cell level with NAD(P)H lifetime shifts. We characterize single cell metabolic heterogeneity in primary human immune cells. We further explore downstream applications including single-cell deposition for integration with single-cell sequencing, and bioreactor integration for long-term, label-free, closed-loop monitoring of cell manufacturing. These capabilities improve the throughput and automation of autofluorescence lifetime measurements of single cell metabolism for applications including immunophenotyping, metabolic screening, and bioprocess monitoring.

Seeing Cells in 3D: Stain-Free Tomographic Flow Cytometry for Deep Live-Cell Phenotyping

Dr. Pietro Ferraro, Director of Research, CNR - ISASI Institute of Applied Sciences and Intelligent Systems -

Traditional flow cytometry (IFC) has revolutionized single-cell analysis by combining the statistical power of flow with the spatial information of microscopy. However, standard IFC remains largely limited to 2D projections and often relies on exogenous labels that can alter cell physiology or limit longitudinal study. This presentation introduces a breakthrough in high-throughput analysis: dye-free tomographic flow cytometry. By integrating holographic optical diffraction tomography (ODT) with controlled microfluidics, we demonstrate the ability to reconstruct the 3D refractive index (RI) distribution of cells in flow. Unlike traditional methods, this approach provides intrinsic quantitative contrast based on the physical properties of cellular organelles without the need for fluorescent labeling. Biomedical case studies will be presented to illustrate the platform's diagnostic and research potential.

Integrating Raman Spectroscopy and Microfluidic Deformation Cytometry for Single-Cell Staging of Barrett’s Oesophagus Progression

Stephen D Evans, PhD, Professor, University of Leeds -

Early detection of oesophageal adenocarcinoma (OAC), which often develops from Barrett’s oesophagus (BO), remains a major clinical challenge due to the lack of sensitive, non-invasive diagnostic tools. This study introduces a multi-modal, label-free approach combining Raman spectroscopy and microfluidic deformation cytometry to characterise biochemical and biomechanical changes in live single cells across disease stages—from healthy oesophageal epithelium to dysplastic BO and OAC. Raman spectroscopy revealed distinct spectral signatures associated with nucleic acids, lipids, and proteins, enabling accurate classification of disease progression using PCA-LDA. Complementary mechano-phenotyping demonstrated increased cellular stiffness in dysplastic and cancerous cells, reflecting cytoskeletal remodelling. Acidic bile salt exposure experiments further highlighted stress-induced biochemical and morphological changes in BO cells, providing insights into microenvironmental influences on disease evolution. By integrating these modalities with machine learning, we hope to establish a high-throughput, non-destructive platform for single-cell staging, offering significant potential for early cancer detection and personalised diagnostics.

3:00pm - 3:30pm
Coffee Break
Grand Ballroom Foyer

Hooke Lecture

Grand Ballroom

How the physical sciences can empower biology : Applications of single molecule fluorescence to the biosciences

Prof Sir David Klenerman, Royal Society GSK Professor, Molecular Medicine, University of Cambridge -

The capability to image single molecules has revolutionised biology. I will explain how these methods work and  how we are currently applying them to study the molecular basis of neurodegenerative disease. Lastly I will describe  how our early single molecule  work on DNA polymerase led to the development of next generation DNA sequencing,  now widely used, and the lessons that can be learnt from this experience. 

4:30pm - 5:15pm
2026 Society Awards Ceremony
Grand Ballroom
5:15pm - 7:00pm
Exhibit Hall Opening Reception
Hall AB
Monday, June 8

Next-Generation Cytometry Technologies Frontier

Grand Ballroom

Microfluidic cytometry for precision cell engineering

Abraham Lee, PhD, Chancellor's Professor, University of California, Irvine -

Microfluidic cytometry processors capable of cell sorting, cell engineering, and cell characterization form the “cell processing units” (CPUs) for bioengineering (bio-CPUs). This is analogous to the CPU (central processing unit) for computer engineering. The current cell engineering paradigm typically focuses on a singular target or pathway, by adding, subtracting or replacing a single genetic code to program cells for targeted diagnosis or therapeutic functions. In reality, the complexity of constantly evolving diseases (cancer, autoimmune diseases, etc.) involves abnormalities that affect the mutated expression of tens of thousands of genes. The ability to sort and engineer cells precisely to program multiple cell functions is critical for the future development of cell therapies. In this talk I will introduce two bio-CPU microfluidic platforms in my lab. 1. Acoustic electric shear orbiting poration (AESOP) device to uniformly deliver genetic cargos into a large population of cells simultaneously. We demonstrate high quality transfected cells with controlled dosage delivery as well as serial delivery of different genetic cargos. These capabilities can be used to optimize the therapeutic efficacy of the engineered cells and also combine it with promising gene editing tools to further condition the cells for more specific in vivo targeting. 2. Arrayed-droplet optical projection tomography (ADOPT) method that relies on flow-controlled, linear cell rotation of live, suspended cells (e.g. immune cells), to allow 360°-imaging of suspension cells with high lateral resolution using simple epifluorescence microscopy. ADOPT is akin to a “bio-GPU” (graphics processing unit) by rapidly producing 3D morphologies of single cells powered by the bio-CPUs. As the AI revolution was accelerated by the advent of the GPUs, it is projected that the bio-GPUs will serve a similar role for the BI (biological intelligence) revolution. A prime application for the bio-CPU and bio-GPUs is immunoengineering that involves the “reprogramming” of the immune system to overcome limitations of the innate or adaptive immune responses that the body naturally produces.

Single-Molecule Sensitive Digital Flow Cytometry

Daniel T. Chiu, PhD, Professor, University Of Washington -

We have developed a multi-parametric high-throughput and high-sensitivity flow-based method for counting single molecules, and applied this method to the analysis of individual extracelluar vesicles and particles (EVPs). EVPs are promising biomarkers but they are highly heterogeneous and comprise a diverse set of surface proteins as well as intra-vesicular cargoes. Yet, current approaches to the study of EVPs lack the necessary sensitivity and precision to fully characterize and understand the make-up and the distribution of various EVP subpopulations that may be present. Digital flow cytometry (dFC) provides single-fluorophore sensitivity and enables multiparameter characterization of EVPs, including single-EVP phenotyping, the absolute quantitation of EVP concentrations, and biomarker copy numbers. dFC has a broad range of applications, from analysis of single EVPs such as exosomes or RNA-binding proteins to the characterization of therapeutic lipid nano¬particles, viruses, and proteins. dFC also provides absolute quantitation of non-EVP samples such as for the quality control of antibodies (Ab), including the concentration of individual and aggregated Ab-dye conjugates and the Ab-to-dye ratio.

10:00am - 10:30am
Coffee Break
Hall AB
10:30am - 11:45am
Parallel Sessions
12:15pm - 1:15pm
Commercial Tutorials

ICCS/ISAC Joint Plenary Session

Grand Ballroom

Rapid-Fire Presentation

Virginia Litwin, PhD, Director, Scientific Affairs, Eurofins Clinical Trial Solutions -

Rapid-Fire Presentation
CLSI H43 Guideline: Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells

Prof. Dr. med. Wolfgang Kern, Executive Management, Internist, Hematologist and Oncologist, Head of business unit Strategy & Business Development, MLL MVZ GmbH -

CLSI H43 Guideline has been finally drafted by an international expert panel and is undergoing review. Targeted time of publication is end of 2026/early 2027. The scope of H43 covers all hematologic neoplasms focusing on differences in flow cytometrically assessible characteristics between these diseases and their normal cell couterparts. Diagnostic and follow-up approaches both will be addressed. General flow cytometric aspects will be discussed using CLSI H62 guideline as the basis. This third edition of CLSI H43 guideline will provide laboratories worldwide applying flow cytometry for hematologic neoplasms with comprehensive, detailed, and ready to use information augmented by educative examples. The draft will benefit from comments provided during the public review period: everyone active in the field is welcome to provide feedback.

Jean Oak, MD, PhD, Clinical Associate Professo, Department of Pathology, Stanford University

Flow Cytometry-Based Monitoring of Chimeric Antigen Receptor (CAR) T Cells: Reagent Selection, Assay Design, and Clinical Utility

Sa Wang, MD, Professor, Department of Hematopathology, Division of Pathology/Lab Medicine, Section Chief, Department of Hospital Flow Cytometry Laboratory, The University of Texas MD Anderson Cancer Center -

Accurate quantification of chimeric antigen receptor (CAR) T cells is essential for monitoring post-infusion CART expansion and persistence and for informing real-time clinical decision-making. Multiparameter flow cytometry (MFC) enables rapid, live-cell detection with absolute quantification and concurrent immunophenotypic characterization. This talk focuses on the practical and technical aspects of flow cytometry–based CAR T-cell monitoring, including selection of CAR detection reagents (target-specific, construct-specific, and target-agnostic strategies), assay optimization, purpose-driven panel design, and matrix-appropriate validation for peripheral blood and other clinically relevant specimens. Assay considerations unique to gene-edited allogeneic CAR T-cell products, including the use of surrogate immunophenotypic approaches when construct-specific reagents are unavailable, will be addressed. The role of MFC in identifying CAR T-cell clonal expansions and in evaluating suspected secondary hematolymphoid neoplasms in the post-CAR T setting will be discussed.

3:15pm - 4:00pm
Coffee Break
Hall AB
4:00pm - 5:00pm
Workshops
5:00pm - 7:00pm
Posters and Exhibit Hall Happy Hour
Hall AB
Tuesday, June 9

RMS/ISAC Joint Imaging and Spatial Biology Frontier

Grand Ballroom

Prof. Dr. Anja Hauser, Professor, Charité Berlin

Prof. Steven Lee, University of Cambridge

10:00am - 10:30am
Coffee Break
Hall AB
10:30am - 11:45am
Parallel Sessions
12:15pm - 1:15pm
Commercial Tutorials

Global Health Plenary

Grand Ballroom

Lucy Ochola, PhD, Institute of Primate Research, Nairobi Kenya

Wiebke Nahrendorf, PhD, University of Edinburgh

3:15pm - 4:00pm
Coffee Break
Hall AB
4:00pm - 5:00pm
Workshops
5:00pm - 7:00pm
Posters and Exhibit Hall Happy Hour
Hall AB
Wednesday, June 10

Bioinformatics, Data, AI Frontier

Grand Ballroom

Yvan Saeys, PhD, Associate Professor, Machine Learning and Systems Immunology, VIB, Ghent University

Raphael Gottardo, PhD, Director, Biomedical Data Sciences Center, CHUV, Lausanne University Hospital

10:00am - 10:30am
Coffee Break
Hall AB
10:30am - 11:45am
Parallel Sessions
12:15pm - 1:15pm
Commercial Tutorials

AAPS/ISAC Joint Translational & Biopharma Plenary

Grand Ballroom

Platelets as Translational Biosensors: Optimised Cytometry and Platelet-Omics Across Diagnosis, Biomarker Discovery, Disease Monitoring and Drug Development

Matthew Linden, PhD, Associate Professor, Haematology and Transfusion Science, The School Biomedical Sciences, University of Western Australia -

Platelets are small, anucleate blood cells with increasingly recognized functional diversity beyond thrombosis and bleeding, including roles in inflammation, immunity, intercellular communication, trauma, and cancer. As an underutilized yet highly accessible translational biosensor, platelets provide reproducible readouts of vascular and megakaryocyte-derived biology that can be leveraged across diagnosis, biomarker discovery, disease monitoring, and drug development. Drawing on collaborative studies, this presentation integrates platelet flow and imaging cytometry with platelet transcriptomics to connect fundamental biology with clinically actionable measurements across health and disease. Emphasis is placed on practical determinants of successful translation, including control of pre-analytical variables and ex vivo activation, assay design and controls, reference materials, and quantitative analysis strategies suited to multicentre studies, clinical trials, and diagnostic implementation. Examples highlight the biological and clinical significance of platelet heterogeneity, functional states, and platelet–leukocyte interactions. Emerging clinical guidelines for platelet flow cytometry will be outlined, focusing on standardization, quality assurance, and near-term opportunities in multiparametric and imaging-enabled phenotyping integrated with platelet-omics.

Brian Eliceiri, PhD, Professor, UC San Diego

3:15pm - 3:45pm
Awards Ceremony
Grand Ballroom
3:45pm - 4:45pm
Business Meeting - Open to All
Grand Ballroom

Closing Reception

Grand Ballroom

After several days of inspiring talks, engaging sessions, and meaningful connections, let’s close out CYTO 2026 in style!

The venue is still to be announced, but you can expect an unforgettable evening filled with great food, drinks, entertainment, and plenty of opportunities to relax, unwind, and reflect on the standout moments of CYTO 2026.